Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain (Mus musculus) |
CD1 | Charles Rivers | ||
| Strain (Mus musculus) | C57Bl/6 | Charles Rivers | ||
| Strain (Mus musculus) | CBA | Charles Rivers | ||
| Genetic reagent (M. musculus) | CBF1-VENUS | Nowotschin et al., 2013 | MGI:5487911 | Dr. Anna-Katerina Hadjantonakis |
| Genetic reagent (M. musculus) |
Rbpj null | Oka et al., 1995 | MGI:1857411 | Dr. Jose Luis de la Pompa |
| Genetic reagent (M. musculus) | Notch1 null | Conlon et al., 1995 | MGI:1857230 | Dr. Jose Luis de la Pompa |
| Genetic reagent (M. musculus) | Tead4 null | Nishioka et al., 2008 | MGI:3770620 | Dr. Hiroshi Sasaki |
| Genetic reagent (M. musculus) | iChr-Control-Mosaic | Pontes-Quero et al., 2017 | MGI:6108166 | Dr. Rui Benedito |
| Genetic reagent (M. musculus) | iChr-Notch-Mosaic | Pontes-Quero et al., 2017 | Dr. Rui Benedito | |
| Genetic reagent (M. musculus) | Polr2a-CreERT2 | Guerra et al., 2003 | MGI:3772332 | Dr. Miguel Torres |
| Cell line (M. musculus) |
iChr-Notch-Mosaic ESC | Pontes-Quero et al., 2017 | Dr. Rui Benedito | |
| Antibody | anti-CDX2 (mouse monoclonal) |
BioGenex | MU392-UC | (1:200) |
| Antibody | anti-CDX2 (rabbit monoclonal) |
Abcam | ab76541 | (1:200) |
| Antibody | anti-YAP (mouse monoclonal) |
Santa Cruz Biotechnology | sc-101199 | (1:200) |
| Antibody | anti-pERM (rabbit polyclonal) | Cell Signalling | 3141 | (1:250) |
| Antibody | anti-E-Cadherin (rat monoclonal) | Sigma | U3254 | (1:250) |
| Antibody | anti-TEAD4 (mouse monoclonal) | Abcam | ab58310 | (1:100) |
| Antibody | anti-DsRed (rabbit polyclonal) | Living Colors, Clontech | 632496 | (1:400) |
| Antibody | anti-GFP (goat polyclonal) | Acris, Origene | R1091P | (1:200) |
| Antibody | anti-HA (rat monoclonal) | Sigma | 11867423001 | (1:200) |
| Recombinant DNA reagent | Tle4-up_H2BmRFP | This paper | modified pBluescript vector | |
| Recombinant DNA reagent | Tbx3-i7_H2BmRFP | This paper | modified pBluescript vector |
|
| Sequence-based reagent | qPCR primers | This paper | SeeSupplementary file 1 | |
| Commercial assay or kit | PicoPure RNA Isolation Kit | ThermoFisher | KIT0204 | |
| Chemical compound, drug | RO4929097 | Selleckchem | S1575 | |
| Chemical compound, drug | Verteporfin | Sigma | SML0534 | |
| Software, algorithm | MINS | Lou et al., 2014 | ||
| Software, algorithm | GraphPad Prism | www.graphpad.com | RRID:SCR_015807 | |
| Software, algorithm | Fiji | fiji.sc | RRID:SCR_002285 | |
| Software, algorithm |
R Project for Statistical Computing |
www.r-project.org | RRID:SCR_001905 |