McLeay 2012.
Methods | Randomised controlled trial (balanced randomised cross‐over design) 30 day washout |
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Participants | Setting: laboratory; New Zealand n = 10 healthy active females mean age 22 (SD 1) years Inclusion/exclusion criteria All participants were physically active and participated in this study. All participants were physically active and participated in recreational level resistance and aerobic‐based exercise at least twice per week. All participants had at least 1 years' experience in training in this manner. Participants completed a Health Screening Questionnaire to exclude those who were at risk physically, culturally or religiously in following the protocol. Those who passed the questionnaire were asked to provide written consent. |
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Interventions |
Intervention Blueberry smoothie 200 g blueberries 50 g banana + 200 mL apple juice. Per 100 mL, total phenolics 168 mg/gallic acid equ.; anthocyanins 96.6 mg; phenolic acid 26 mg, flavonoids 10.2 mg; vitamin C 45 mg; vitamin E 3 mg Placebo Smoothie 25 g dextrose 50 g banana + 200 mL apple juice Duration 4 days |
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Outcomes |
PRIMARY Delayed onset muscle soreness was recorded using a subjective 10‐point scale where 0 is "no soreness" and 10 is "very, very painful." Participants were asked to step up (concentric muscle action) onto a 40 cm box then step down (eccentric muscular contraction) and the soreness was rated. SECONDARY Muscle function was tested using an isokinetic dynamometer (Biodex Medical Systems Inc. 2004). Range of motion of the leg was set at 60 degrees for concentric and eccentric contractions and at 75 degrees for isometric contractions. The participants performed 5 maximal contractions of each type with each set separated by 2 minutes of recovery. Concentric and eccentric torque was measured at an angular velocity of 30 degrees per second. |
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Exercise type | 3 sets of 100 eccentric repetitions of the quadriceps | |
Sources of funding | Funded by an Institute of Food, Nutrition and Human Health Postgraduate Research Award, and the New Zealand Ministry of Science and Innovation, contract C06X0807 awarded to Plant and Food research Ltd. | |
Notes | Drinks consumed on day of exercise the again at 12 hours and 36 hours post‐exercise | |
Risk of bias | ||
Bias | Authors' judgement | Support for judgement |
Random sequence generation (selection bias) | Low risk | Author response: "A table with vertically numbered cells 1‐10 had either ‘blueberries’ or ‘no blueberries’ (4 of each) next to them. Names were then simply pulled from a hat and typed into these cells in drawn order. A simple method, yet allowed for randomised allocation of treatment." |
Allocation concealment (selection bias) | High risk | No details in manuscript Authors were contacted on 27 May 2016 via email Author response: "Due to the form the treatment was given as (smoothie), we were unable to blind the study. Both control and blueberry smoothies were isocaloric however. The study was a cross‐over design and both treatment and leg of damage was randomised." Adequate safeguards not reported for the first phase; the second phase was predictable |
Blinding of participants and personnel (performance bias) All outcomes | High risk | Not blinded |
Blinding of outcome assessment (detection bias) All outcomes | High risk | Probably not blinded |
Incomplete outcome data (attrition bias) All outcomes | Low risk | All participants completed the study with no adverse effects |
Selective reporting (reporting bias) | Low risk | No published protocol available All outcomes reported at all time points Manuscript states: "there were no reported adverse effects from the dietary intervention" |
Other bias | Unclear risk | Participants were asked to complete a food diary but no detail on whether participants were asked to refrain from using anti‐inflammatory drugs or other supplements that could reduce soreness |