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. 2017 Dec 12;2017(12):CD003633. doi: 10.1002/14651858.CD003633.pub3

Wissel 2001.

Methods Randomised, double‐blind, parallel design
Randomisation: method not described
Setting: multicentre (Austria and Czech Republic)
Duration: 4 weeks, follow‐up up to 16 weeks
Participants 68 participants enrolled (BtA group = 35; placebo group = 33)
% Female: BtA: 46%; placebo: 56%
Mean age, SD: BtA: 45.8 years, 13.2; placebo: 49.7 years, 9.6
Mean CD duration, SD: BtA: 6.5 years, 8.0; placebo: 4.8 years, 4.4
Mean CD severity, SD (Tsui scale): BtA: 11.1, 1.7; placebo: 11.5, 1.8
Inclusion criteria:
  • moderate or severe CD (Tsui score ≥ 9)


Exclusion criteria:
  • pure anterocollis

  • treatment with BtA in the last 12 weeks

  • last BtA dose > 750 U (Dysport) or < 250 U (Dysport)

  • lack of response to previous BtA treatments

  • complex pattern of CD requiring EMG assistance or injection of > 3 muscles

Interventions BtA: Dysport (abobotulinumtoxinA); 500 U, diluted with 1 mL 0.9% saline solution
Placebo: 0.125 mg of human serum albumin and 2.5 mg of lactose, diluted with 1 mL 0.9% saline solution
Study drug preparation: BtA and placebo provided in vials by Ipsen
Muscles injected: based on clinical assessment 2 or 3 muscles were selected for injection: sternocleidomastoid (100 U‐200 U), splenius capitis (250 U‐350 U), trapezius (100 U‐200 U), and levator scapulae (100 U‐200 U). Each muscle was injected in 2 sites
EMG guidance: no
BtA dose per participant: 500 U
Outcomes Primary outcome:
  • Tsui Scale score


Secondary outcomes:
  • Pain Assessment (4‐point scale: none, mild, moderate, severe)

  • Physician Global Assessment of Change (5‐point scale: worse, no improvement, improvement < 50%, improvement > 50%, symptom free)

  • Patient Global Assessment of Change (5‐point scale: worse, no improvement, improvement < 50%, improvement > 50%, symptom free)

  • Clinical Global Assessment (taking into account efficacy and safety)

  • Adverse effects

Notes Participants were withdrawn from the study if they were considered non‐responders at week 4. Participants with an ongoing response at weeks 4 and 8 continued until re‐treatment was required.
Study discontinuations (at week 4), reasons:
BtA: n = 0
Placebo: n = 0
Risk of bias
Bias Authors' judgement Support for judgement
Random sequence generation (selection bias) Unclear risk Quote: "Patients were randomly assigned to receive either placebo or 500 units of Dysport"
 Comment: insufficient information about the method of randomisation to permit judgement of low risk or high risk
Allocation concealment (selection bias) Unclear risk Comment: insufficient information to permit judgement of low risk or high risk
Blinding of participants and personnel (performance bias) 
 All outcomes Low risk Quote: "Blinded study medication was supplied (...) as identical vials containing either Dysport (...) or placebo"
Blinding of outcome assessment (detection bias) 
 Objective outcomes Unclear risk Comment: insufficient information to permit judgement of low or high risk
Blinding of outcome assessment (detection bias) 
 Subjective outcomes Unclear risk Comment: although placebo was identical to intervention, the fact that most of the participants had previously been treated with Bt could have led to a degree of bias.
Incomplete outcome data (attrition bias) 
 All outcomes Low risk Quote: "In order to remove the bias created by the withdrawal of the majority of placebo patients at week 4, a last observation carried forward technique was used for the week 8 analyses"
Selective reporting (reporting bias) Low risk Comment: the expected outcomes that are usually evaluated in intervention trials for this condition were reported in this study.
For‐profit bias High risk Quote: "Blinded study medication was supplied by Ipsen Ltd"
Enriched population – preferential enrolment of positive responders High risk Comment: out of the 68 participants enrolled, 47 had received BtA injections previously
Enriched population – exclusion of poor responders High risk Quote: "Patients with pure anterocollis were excluded"

Bt: botulinum toxin
 BtA: botulinum toxin type A
 CD: cervical dystonia
 CDSS: Cervical Dystonia Severity Scale
 F/U: follow‐up
 GAS: Global Assessment Scale
 IGAE: Investigator Global Assessment of Efficacy
 PEGR: Patient Evaluation of Global Response
 TWSTRS: Toronto Western Spasmodic Torticollis Rating Scale
 VAS: visual analogue scale