Salmanpour 2006

Methods	Study design: randomised controlled trial Setting/location: Dermatology Clinic of Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran Study period: unclear Sample size calculation: not reported
Participants	Type of Leishmania: not reported Inclusion criteria: participant with CL confirmed Exclusion criteria: not described N randomised: 60 Withdrawals: 0 N assessed: 60 participants distributed into the 3 groups of equal number (n = 20) Age: not reported Sex: not reported Baseline data: lesions were located on the head, neck and lower and upper extremities. Lesion duration was divided in 2 groups: a duration of < 3 months and > 3 months. Regarding lesion size, the authors divided the sizes in 3 groups: < 1 cm, 1‐3 cm and > 3 cm.
Interventions	Type of interventions: Group 1: cryotherapy. The freezing time was 10‐30 s with a thawing interval of 20 s Group 2: cryotherapy + ILMA. The participants first received cryotherapy and after 5‐10 min were given ILMA Group 3: ILMA. The solution was injected into each lesion (0.2 to 1.5 cm³ per session per week, depending on the size) Duration of intervention: weekly for a total of 6‐8 times for each case
Outcomes	Healing rates: percentage of participants 'cured': timing not reported Adverse effects Time points reported: not reported
Notes	Study funding sources: not reported Possible conflicts of interest: none declared
Risk of bias
Bias	Authors' judgement	Support for judgement
Random sequence generation (selection bias)	Unclear risk	Comment: insufficient detail was reported about the method used to generate the allocation sequence.
Allocation concealment (selection bias)	Unclear risk	Insufficient information to permit judgment
Blinding of participants and personnel (performance bias) All outcomes	High risk	The outcome is likely to be influenced by lack of blinding.
Blinding of outcome assessment (detection bias) All outcomes	Unclear risk	No blinding of outcome assessment, and the outcome measurement is likely to be influenced by lack of blinding.
Incomplete outcome data (attrition bias) All outcomes	High risk	No explanation was provided for missing outcome data.
Selective reporting (reporting bias)	Low risk	The study protocol is not available, but it is clear that the published reports included all expected outcomes, including those that were pre‐specified.
Other bias	High risk	Sample size calculation, reporting of Leishmania spp involved and baseline comparability were not correctly reported