Figure 2. The Rag-mTORC1 pathway negatively regulates myogenesis in vivo.

TA muscles were coinjected with BaCl₂ and lentiviruses expressing shRagA (A), shRaptor (B), or shScramble (control). Injected muscles were isolated on day 7 after injury and subjected to measurement of CSA of regenerating myofibers (n = 7–8). Data were presented as the size distribution of all myofibers (left panels) and average CSA (right graphs). *P < 0.05, **P < 0.01 by 2-tailed paired t test. All error bars represent SEM.