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. 2019 Apr 2;129(5):1926–1939. doi: 10.1172/JCI99550

Figure 2. IL-26 colocalization with CD4 and CD8.

Figure 2

(A) Immunofluorescence of IL-26 (green), CD4 (red), and nuclei (DAPI, blue) in T-lep and L-lep lesions. Data are representative of 4 individual samples. Original magnification, ×20. Scale bars: 10 μm (enlarged insets). (B) Colocalization of CD4 and IL-26, as determined by ImageJ. Data represent the mean ± SEM (n = 4). **P < 0.01, by 2-tailed Student’s t test. (C) Immunofluorescence of IL-26 (green), CD8 (red), and nuclei (DAPI, blue) in T-lep and L-lep lesions. White arrows indicate colocalization of green and red (yielding yellow). Original magnification, ×20. Scale bars: 10 μm (enlarged insets). (D) Colocalization of CD8 and IL-26, as determined by ImageJ. Data represent the mean ± SEM (n = 4). *P < 0.05, by 2-tailed Student’s t test. (E) Comparison of CD4 or CD8 colocalization with IL-26 in T-lep using ImageJ. Data represent the mean ± SEM (n = 4). *P < 0.05, by 2-tailed Student’s t test.