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. 2019 Apr 2;129(5):1926–1939. doi: 10.1172/JCI99550

Figure 3. IL-26 colocalization with CD68+ macrophages in leprosy lesions.

Figure 3

(A) Immunofluorescence of IL-26 (green), CD68 (red), and nuclei (DAPI, blue) in T-lep and L-lep lesions. White arrows indicate colocalization of IL-26 and CD68. Data are from 5 individual samples. Original magnification, ×20. Scale bars: 10 μm (enlarged insets). (B) Colocalization of IL-26 and CD68 in T-lep and L-lep lesions using ImageJ. Data represent the mean ± SEM (n = 5). *P < 0.05, by 2-tailed Student’s t test. (C) Immunofluorescence of IL-26 (green), CD68 (red), CD4 (cyan), and nuclei (DAPI, blue) in T-lep and L-lep lesions. Data are from 3 individual samples. White arrows indicate colocalization of IL-26 (green) and CD68 (red) (yielding yellow). Original magnification, ×20. Scale bars: 10 μm (enlarged insets). (D) Comparison of CD4 or CD68 colocalization with IL-26 in T-lep using ImageJ. Data represent the mean ± SEM (n = 4). *P < 0.05, by 2-tailed Student’s t test.