Skip to main content
. 2019 Apr 2;129(5):1926–1939. doi: 10.1172/JCI99550

Figure 5. IL-26 has direct antimicrobial activity against mycobacteria.

Figure 5

(A) M. leprae was cultured for 4 days with increasing concentrations of IL-26. Rifampicin (Rif) was used as a positive control. Viability of M. leprae was calculated by the ratio of bacterial 16S rRNA and RLEP DNA detected by qPCR. Relative viability was determined by comparing the treatment ratio with the media ratio. Data represent the mean ± SEM (n = 5). (B) M. leprae (mLEP) was cultured for 4 days with 10 μM native IL-26 or denatured IL-26 (dIL-26). Rifampicin was used as a positive control. Viability of M. leprae was calculated according to the ratio of bacterial 16S rRNA and RLEP DNA detected by qPCR. Relative viability was determined by comparing the treatment ratio with the media ratio. Data represent the mean ± SEM (n = 5). (C) M. tuberculosis (mTB H37Ra) was cultured for 4 days with increasing concentrations of IL-26. Rifampicin was used as a positive control. A CFU assay was performed and numbers quantified after 3 weeks. Data represent the mean ± SEM (n = 5). *P < 0.05 and **P < 0.01, by repeated-measures 1-way ANOVA.