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. 2019 Apr 2;129(5):1926–1939. doi: 10.1172/JCI99550

Figure 8. IL-26 induces antimicrobial activity against intracellular bacteria.

Figure 8

(A) MDMs were treated with IL-26 for 30 minutes and then infected with M. leprae for 4 days. Rifampicin was used as a positive control. Viability of M. leprae was measured by qPCR (n = 6). (B) MDMs were treated with IL-26 or denatured IL-26 and then infected with M. leprae, and bacterial viability was measured as in A (n = 4). (C) MDMs were treated with IL-26 or denatured IL-26 and then infected with M. tuberculosis (H37Ra) as in A. Viability of intracellular M. tuberculosis was determined by CFU assay (n = 3). (D) MDMs were treated with IL-26 for 4 hours and then washed and infected with S. aureus for 4 hours, followed by removal of the extracellular bacteria. After overnight culture, the viability of intracellular S. aureus was determined by CFU assay (n = 5). (E) MDMs were treated with IL-26 with or without wortmannin (Wm) and then infected with M. leprae, and bacterial viability was measured as in A (n = 6). *P < 0.05 and **P < 0.01, by repeated-measures 1-way ANOVA. All data represent the mean ± SEM.