Figure 3.

G₀ NSCs Are Prevalent in the Dorsal Nervous System

(A) The distribution of G₀-quiescent NSCs (red) and G₂-quiescent NSCs (cyan) in each hemi-segment of the tVNC. Dotted line indicates ventral midline. G₀ NSCs are prevalent in dorsal regions, and G₂ NSCs in ventral regions. A, anterior; P, posterior. G₀ and G₂ NSCs according to Otsuki and Brand (2018).

(B) A single hemi-segment of a tVNC in the same orientation and colors as in (A). G₀ NSCs are circled. Dotted line indicates ventral midline. To enable comparison with (A), the G₀ NSCs NB2-2 and NB3-4 are indicated. Maximum intensity projection.

(C) Comparison of the numbers of G₀- versus G₂-quiescent NSCs per hemi-segment in ventral (Vnd⁺), intermediate (Ind⁺), and dorsal (Msh⁺) regions. Data assembled using data from Chu et al., 1998, D'Alessio and Frasch, 1996, Isshiki et al., 1997, McDonald et al., 1998; and Weiss et al. (1998).

(D) The same tVNC hemi-segment as in (B), with Msh-expressing regions labeled in green using G-TRACE. G₀ NSCs are circled. Most G₀ NSCs reside in the Msh⁺ domain. To enable comparison with (A), the G₀ NSCs NB2-2 and NB3-4 (that do not express Msh) are indicated. Maximum intensity projection.

Anterior is up, and dorsal is right in all images.

See also Figure S3.