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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Virology. 2019 Mar 4;531:100–113. doi: 10.1016/j.virol.2019.03.002

Fig. 5. Replication capacity of virion-associated HDV RNA genomes harvested from woodchucks M6593 and F6438.

Fig. 5.

The replication capacity of HDV genomes was analyzed using transfection of HDV RNA isolated from serum virions into Ag-293 cells. The details are described in Materials and Methods. The left Y axis reflects serum concentration of HDV (black rectangles) in logarithmic scale. The right Y axis represents the values of replication capacity, which is a normalized parameter that shows how many new HDV genomes/cell were produced as a result of transfection per each HDV genome used per cell in the transfection mixture. In vitro transcribed and gel-purified unit length HDV RNA (58) was used a positive control. The level of the replication capacity measured for this control RNA was considered as 100% value. The values of the replication capacity (%) are shown as blue (M6593) or yellow (F6438) bars. The X axis represents the time (week) after super-infection with HDV. The top panel shows the results for woodchuck M6593, while the bottom panel - for woodchuck F6438.