Figure 3.

Application of TT filter (blue) and TA filter (red) to 3.9 × 4.1 mm² sections of infarcted rabbit tissue where infarct is located at the bottom of the image, indicated by severe fibrosis. (A) A raw confocal tile scan exhibiting varying degrees of T-system remodeling is shown. (B and C) Smoothed TT and TA filter detections are shown with heatmaps normalized to maximal density of filter detections. A threshold was applied to the smoothed TA hits to exclude blood vessels. Striations arise from close apposition of punctate TT patterns typically observed in rabbit cardiomyocytes (43). As summarized in Table 1, the distal region presented 18.9 ± 1.0% TT detection compared to 10.1 ± 0.8% in the proximal region. TA detection paralleled TT detection, with the distal region exhibiting 9.5 ± 2.0% and the proximal region exhibiting 16.0 ± 2.0% TA detection. To see this figure in color, go online.