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. 2019 Mar 28;116(8):1456–1468. doi: 10.1016/j.bpj.2019.03.016

Figure 1.

Figure 1

Effect of MβCD-mediated cholesterol depletion on membrane topology. (a) Left: representative fluorescence images of Filipin III-stained control and MβCD-treated HeLa cells are shown. Right: shown is a box plot of mean intensity of Filipin III in cells in mentioned conditions (N = 30 cells each). Center lines of boxes show the medians; stars show the means; box limits indicate the 25th and 75th percentiles; and whiskers extend 1.5 times the interquartile range from the 25th to 75th percentiles. ∗∗p < 0.001, Mann-Whitney U test. (b) Shown are representative DIC (first), IRM (second) images, kymographs of regions overlaid on the images in red (third, scale bars in y, 16 s, scale bars in x, 5 μm) and corresponding SDtime maps of control versus MβCD-treated cells (non-FBRs blacked out). Scale bars, 10 μm. See Table S3 for statistics. To see this figure in color, go online.