Figure 2.

Cholesterol depletion by MβCD reduces temporal fluctuations and enhances membrane tension. (a) Shown are averaged PSDs of FBRs in MβCD-treated cells and their controls (solid lines) with their backgrounds (dashed lines); inset shows f (ratio of background subtracted PSDs). (b) Left: Shown are box plots of $\overline{\sigma \left(f_1,f_2\right)}$ in two different frequency regimes. Right: exponent in the two conditions is shown. N = 70 cells each. (c) Left: single FBR statistics of SD_space in control and cholesterol-depleted cells. n_control = 1683 FBRs, n_MβCD = 1471 FBRs, and N = 70 cell each. Right: correlation lengths are shown. (d) Single cell statistics of SD_space (left) and correlation lengths (right) in the two conditions are shown. N = 70 cell each. (e) Shown are box plots of mechanical parameters A, η_eff, γ, and σ in control and MβCD-treated cells. N = 70 cell each. ^∗p < 0.05, ^∗∗p < 0.001, Mann-Whitney U test. See Fig. S1 and Document S1. Supporting Discussion, Figs. S1–S10, and Tables S1–S2, Table S3. Statistical Parameters for Data Presented in Main Figures for statistics. To see this figure in color, go online.