Figure 4.

Enhanced spatial and mechanical intracellular heterogeneity on MβCD treatment. (a) Intra-FBR fluctuations heterogeneity measured by SD (SD_time) for the two conditions are shown. (b) Shown are intracellular long-range fluctuations heterogeneity measured by box plots of the number of dissimilar FBR pairs. Dissimilarity is evaluated by comparing SD_time. N = 70 cells each. (c) Lines in magenta and black connect FBRs that are dissimilar and similar in SD values, respectively. The lines are overlaid on the SD_time maps in representative control and MβCD-treated cells. Each node in the maps represents the center of an FBR. Scale bars, 10 μm. (d) Shown are histograms and box plots of intracellular heterogeneity of cell averaged SD_time, SD_space, and σ in the two conditions. N = 70 cells each. (e) Representative single pixel maps of tension (σ) and goodness of fit (R²) for a control and MβCD-treated cell are shown. The white dashed line marks the boundary of the cell. Fitting was performed for pixels inside this boundary. Scale bars, 10 μm. ^∗p < 0.05, ^∗∗p < 0.001, ns p > 0.05, Mann-Whitney U test. See Figs. S1 and S4 and Document S1. Supporting Discussion, Figs. S1–S10, and Tables S1–S2, Table S3. Statistical Parameters for Data Presented in Main Figures for statistics. To see this figure in color, go online.