Fig. 1.

Total differentiate adipocyte enumeration by flow cytometry. hMSCs treated with C. ficifolia extracts were differentiated and quantified by flow cytometry. Cells were with various concentrations (1.1 µg/mL to 18 µg/mL) of three different solvent-free extracts. (a) Differentiated DMSO control and undifferentiated negative control. (b) The histograms represent the differentiated cells that were stained with Nile red dye and analyzed in the FL1 channel, background normalized with undifferentiated control, quantitative comparison of each extracts with DMSO control, the data represent in percentage (%). The P-values were determined using ANOVA (RM one-way ANOVA, with the Greenhouse-Geisser corrections). Data are presented as mean ± S.E., n = 3. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.