Figure 2.

CDKi inhibits RNA Polymerase CTD Phosphorylation. (A) Co-IP showing AML-ETO (AE) fusion protein from Kasumi-1 cells extracts was precipitated using anti-CDK9. Asterix (*) depicts non-specific bands. (B) CDKi treated Kasumi-1 cell extracts were tested for phospho-Ser2 RNA polymerase II levels by western blot. (C, D) THZ1 treated Kasumi-1 cell extracts from the indicated time-course experiment were tested for Ser5 and Ser7 phosphorylated RNA polymerase II levels by western blot. (E) Quantitation of Annexin V/PI staining analysis of Kasumi-1 cells treated with THZ1 at 400 nM for 30, 60 120 and 240 min. Asterisk (*) indicate P-values <0.05