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. 2019 Jan 30;47(8):3996–4010. doi: 10.1093/nar/gkz054

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — SDE2 cleavage exposes an N-degron required for the Arg/N-end rule. (A) Schematic of SDE2 cleavage at a diglycine motif generating SDE2^Ct with its Nt Lys⁷⁸ exposed. (B) U2OS cells expressing wild-type (WT) or G76A/G77A full-length SDE2-Flag were treated with 50 μg/ml cycloheximide (CHX) or DMSO (vehicle) for the indicated times and analyzed by Western blotting (WB). (C–E) CHX chase and WB analysis of U2OS cells expressing either (C) WT or K78V full-length SDE2-Flag, or (D) WT full-length SDE2-Flag versus HA-SDE2^Ct-Flag, or (E) WT full-length SDE2-Flag versus Met-SDE2^Ct-Flag. Representative images are shown, and quantification graphs can be found in Supplementary Figure S1.