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Extraction of the ribosome exit tunnel coordinates. For a given structure of the ribosome large subunit (LSU) (from Schmidt et al. (97)), we first locate the PTC and then apply a tunnel search algorithm (26) to reconstruct the geometry of the exit tunnel (more details in ‘Materials and methods’ section). The inset in the right panel shows the exit tunnel with the PTC and the constriction site that separates the tunnel between its upper and lower parts.
