Table 2.

Modification frequencies of all methylated residues in 23S rRNA from wild-type and Δmtn strains

Modification	Enzyme	WT 50S	Δmtn 45S	Δmtn 50S
m1G745	RlmA	100	100	100
m5U747	RlmC	100	60 ± 16	61 ± 13
m6A1618	RlmF	100	100	100
m2G1835	RlmG	100	100	100
m3Ψ1915	RlmH	96 ± 1.4	30 ± 14	86 ± 2.9
m5U1939	RlmD	98 ± 0.3	81 ± 3.5	98 ± 0.8
m5C1962	RlmI	100	100	100
m6A2030	RlmJ	97 ± 0.9	94 ± 1.4	94 ± 0.2
m7G2069	RlmKL	100	96 ± 1.7	96 ± 2.3
Gm2251	RlmB	99 ± 0.4	96 ± 2.2	93 ± 0.5
m2G2445	RlmKL	100	100	100
Cm2498	RlmM	97 ± 0.2	96.1±0.4	98 ± 0.2
m2A2503	RlmN	100	100	100
Um2552	RlmE	91 ± 5.2	28 ± 16	81 ± 8.7

The frequency of each modification as indicated on the right was calculated from the XIC peak intensity ratio between methylated and unmethylated RNA fragments. Data represent average values ± SD from biological triplicates. Bold text represents modification frequencies that are decreased by a statistically significant amount in Δmtn strains (P < 0.05).