Figure 1.

Lack of Pol ϵ catalytic domains (pol2-16) leads to replication stress. (A) Flow cytometry profiles of log phase yeast cultures of wild type and pol2-16 strains used for dNTP pool measurements; (B) intracellular dNTP levels; presented as mean values ± SD (n = 3); (C) western blot detection of Sml1 levels in whole cell extracts of the wild type, pol2-16 and wild type strains treated with the 4-nitroquinoline 1-oxide (4-NQO) at 0.2 μg/ml for 4 h, representative of two independent measurements is presented.