Table 2.
Mutation rates of specific mutation types detected in Canr yeast colonies
| Type of mutation/ Strain | WT | pol2-16 | rev3Δ | pol2-16 rev3Δ | pol2-16 rev3Δ msh6Δ | pol2-16 pol3L612M | pol3L612M |
|---|---|---|---|---|---|---|---|
| Base substitutions | 2.88 a (113) b | 82.28 (190) | 1.58 (87) | 29.58 (113) | 285.22 (164) | 368.66 (115) | 18.7 (118) |
| Transitions | 0.99 (39) | 25.98 (60) | 0.85 (47) | 18.59 (71) | 205.22 (118) | 182.73 (57) | 12.68 (80) |
| AT→GC | 0.1 (4) | 13.86 (32) | 0.11 (6) | 11.78 (45) | 76.52 (44) | 57.7 (18) | 5.55 (35) |
| GC→AT | 0.89 (35) | 12.13 (28) | 0.75 (41) | 6.81 (26) | 128.7 (74) | 125.02 (39) | 7.13 (45) |
| Transversions | 1.88 (74) | 56.3 (130) | 0.73 (40) | 10.99 (42) | 80 (46) | 185.93 (58) | 6.02 (38) |
| AT→CG | 0.23 (9) | 3.9 (9) | 0.02 (1) | 1.57 (6) | 3.48 (2) | 32.06 (10) | 1.9 (12) |
| AT→TA | 0.23 (9) | 17.32 (40) | 0.07 (4) | 1.31 (5) | 1.74 (1) | 32.06 (10) | 1.27 (8) |
| GC→TA | 0.64 (25) | 12.13 (28) | 0.58 (32) | 7.85 (30) | 74.78 (43) | 102.58 (32) | 2.22 (14) |
| GC→CG | 0.79 (31) | 22.95 (53) | 0.05 (3) | 0.26 (1) | <1.74 (<1) | 19.23 (6) | 0.63 (4) |
| InDels c | 0.99 (39) | 22.95 (53) | 0.4 (22) | 16.75 (64) | 31.3 (18) | 298.13 (93) | 9.83 (62) |
| + 1 | 0.31 (12) | 4.76 (11) | 0.04 (2) | 3.66 (14) | 12.17 (7) | 48.09 (15) | 1.27 (8) |
| − 1 | 0.69 (27) | 18.19 (42) | 0.36 (20) | 13.09 (50) | 19.13 (11) | 250.05 (78) | 8.56 (54) |
| Insertions ≥2 | 0.08 (3) | 0.43 (1) | 0.07 (4) | 0.79 (3) | 1.74 (1) | <3.21 (<1) | 0.16 (1) |
| Deletions ≥2 | 0.15 (6) | 0.87 (2) | 0.35 (19) | 1.57 (6) | 1.74 (1) | <3.21 (<1) | 0.16 (1) |
| TBPS d | <0.03 (<1) | 1.3 (3) | 0.02 (<1) | 1.31 (5) | <1.74 (<1) | <3.21 (<1) | 0.16 (1) |
| Complex c | <0.03 (<1) | 2.17 (5) | <0.02 (<1) | <0.26 (<1) | <1.74 (<1) | 3.21 (1) | <0.16 (<1) |
| Total | 4.1 (161) | 110 (254) | 2.4 (132) | 50 (191) | 320 (184) | 670 (209) | 29 (183) |
aRates [Canr x 10−7] for particular types of mutations were calculated as described previously (24);
bNumber of events for specific classes of mutations are shown in brackets;
cIndels include minus and plus one nucleotide mutations;
dTBPS are tandem base pair substitutions;
eComplex mutations are defined as multiple changes within short DNA stretches (separated by up to 10 nt).
5% or less of sequenced Canr yeast colonies were wild type and they were not included in the analysis.