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. 2019 Feb 18;47(8):e45. doi: 10.1093/nar/gkz096

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — In vitro assays of a novel driver gene NPAT predicted uniquely by DriverML. (A) The expression of NPAT in H520 and H1703 lung cancer cells transfected with siRNA by real-time PCR. (B) CCK-8 cell proliferation assay for lung cancer cells transfected with siRNA. (C) Invasion assay following knockdown of NPAT in lung cancer cells. (D) Colony formation assay in lung cancer cells transfected with NPAT siRNA or control siRNA. (E) Cell cycle profile of control and NPAT knockdown cells. (F) Western blot analysis of protein makers related to cell cycle in control and NPAT knockdown cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein is used as control. All cell assays were performed in triplicate. The error bars indicate SD of three independent experiments. **P < 0.01, ***P < 0.001 using the two-sided Student’s t test.