Fig. 1.

In-vivo RNAi screen for factors regulating body size. a RNAi screen of 1845 genes of the Drosophila secretome and receptome selected by gene ontology analysis. Upstream activating sequence (UAS)-inducible RNAi constructs against these genes were expressed ubiquitously using da-GAL4, and pupal sizes were determined (data are presented in Supplementary Data 1). b, c Distribution of the pupal sizes from the screen (b) revealed 89 gene hits that resulted in a significant increase (c: blue, top) or decrease (c: orange, bottom) in body size (a Z-score > ± 2 was used as a cutoff) by comparison with the mean of all lines. n = 9–55. d Genes and pathways identified in the screen having known size-regulatory function, as well as breathless (btl), are marked with arrows. Error bars indicate SEM. Underlying data are provided in Supplementary Data 1