Figure 8.

In situ localization of PsN5-10 and PsN120-1 mRNA in effective pea cv Sparkle nodules and ineffective nodules on the mutant E135. Nodules were harvested from 4-week-old plants. Bacterial nifH and ropA genes were used for definition of meristem (I), infection zone II (II), interzone II to III (II–III), and nitrogen fixation zone III (III) in the nodules. Longitudinal sections (10 μm) through the nodules were hybridized with digoxigenin-labeled antisense probes. Hybridization signals are visible as purple or blue. The bar represents 400 μm and all micrographs were taken at the same magnification.