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. 2019 Apr 26;10:1943. doi: 10.1038/s41467-019-09914-3

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019 2019

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Fig. 4 — Seroreactivity of Zika virus (ZIKV)- and Dengue virus (DENV)-infected samples with ZIKV peptides. a End-point titers of various acute (Acute-V0; orange symbols and Acute-V28; green symbols) and convalescent ZIKV (ZIKV-Conv; magenta symbols) and convalescent DENV human serum samples (DENV; blue symbols) tested for binding to various ZIKV antigenic site peptides in ELISA are depicted. ELISA was performed with fivefold serially diluted (starting at 1:100) samples. One-way ANOVA was performed with a Bonferroni post-hoc analysis. ****p < 0.0001, **p < 0.005 and *p < 0.05. b Table showing frequency and percentage of samples with end-point titers of ≥100 for each of the sample groups; ZIKV-Acute-V0 and 28, ZIKV-Conv and DENV for each ZIKV peptide determined by peptide ELISA. Frequency and percentage seropositivity were calculated for 19 samples each of ZIKV-Acute-V0 and V28, 13 samples for ZIKV-Conv, and 27 samples for convalescent DENV-infected samples