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. 2019 Apr 26;10:1943. doi: 10.1038/s41467-019-09914-3

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Antibody affinity maturation in Zika virus (ZIKV)-infected serum and urine to NS1, E, E-dIII. SPR analysis of individual sera (a, c, e) or urine (b, d, f) post-ZIKV infection was performed with purified recombinant NS1 (a, b), ZIKV-E (c, d), and ZIKV-E-domain III (e, f) proteins to determine the dissociation kinetics (off-rates) at different time points post infection. Antibody off-rate constants that describe the fraction of antibody–antigen complexes decaying per second were determined directly from the serum/urine sample interaction with recombinant and NS1, ZIKV-E, ZIKV-E-domain III proteins using SPR in the dissociation phase as described in Methods. Mean values with standard deviation are shown by bar for each group. Antibody off-rates were not calculated for samples with total antibody binding (Max RU shown in Fig. 5) of <10 at 10-fold dilution and is not shown in figure. The statistical significances between each time point (visit days) were determined using two-tailed paired t-test in GraphPad software. p-Values <0.05 were considered significant with a 95% confidence interval. Statistically significant with p-values of <0.005 (**), or <0.001 (***) are shown. Source data are provided as a Source Data file