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. 2019 Apr 26;10:1944. doi: 10.1038/s41467-019-09770-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Cardiac-projecting neurons in stellate ganglia (SG) are clustered craniomedially. a A 3D projection (1200 µm z-stack) of the dorsal side of a C57BL/6J mouse heart whole-mount stained with TH (green). b Cartoon depicting cholera toxin subunit B (CTB)-Alexa Fluor 488 (CTB-488) injections into the heart to retrogradely trace neurons in the paravertebral chain ganglia that project to the heart. c A MIP image of the left paravertebral chain from the middle cervical ganglion (MCG) to the second thoracic ganglion (T2G) showing the location of CTB-488+ neurons that project to the heart. d Summary heat map of the right stellate ganglion (RSG) and the left stellate ganglion (LSG). n = 5 mice (d). Scale bars are 1 mm (a, c). CTB injections were performed in C57BL/6J mice