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. 2019 Apr 26;10:1935. doi: 10.1038/s41467-019-09970-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A fraction of CSF-1R is located in the nucleus of human monocytes. a Sorted peripheral blood human monocytes were stained with an anti-CSF-1R antibody (Cter sc-692) or a control IgG (green) and Dapi (blue), followed by confocal imaging analysis (n = 3, scale: 10 μm). b Quantification of the signal generated by CSF-1R labeling (green) and Dapi (blue) according to indicated axes (A.U.: arbitrary units, ROI: region of interest, scale: 10 μm). c Monocytes were stained with an anti-CSF-1R antibody (Cter sc-692) and Dapi (blue), followed by confocal imaging analysis (stack of 50 pictures of 0.2 μm) to reconstitute an orthogonal view (scale: 10 μm). d The specificity of CSF-1R labeling with sc-692 antibody (Ab in green) was explored in monocytes transfected 24 h before with a CSF-1R specific or a control si-RNA and treated or not with sc-692 blocking peptide (BP, n = 2, scale: 10 μm, Dapi in blue). e Monocytes were fractionated into cytoplasmic plus membrane (C+M) and nuclear (N) fractions and analyzed by immunoblotting with antibodies that recognize CSF-1R (Cell signaling #3152), Lamin B (N fraction) and actin (C+M fraction) (n = 3). f Immunogold analysis of CSF-1R expression in monocytes using the anti-Cter sc-692 antibody and electron microscopy (n = 2, scale: 500 nm; insert: 100 nm)