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. 2019 Apr 26;10:1935. doi: 10.1038/s41467-019-09970-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — CSF-1R interacts with EGR1 to modulate gene expression. a Co-immunoprecipitation experiment using an anti-CSF-1R (sc-365719) or a control IgG in monocytes followed by immunoblotting with anti-CSF-1R and anti-EGR1 antibodies (n = 3). Asterisk (*) indicates a longer exposure of the blot. b Venn diagram of peak calling (input normalization) common to three donors from ChIP-seq experiment with sc-46662 anti-CSF-1R and sc-110X anti-EGR1 antibodies. c Ranking heatmap centered on CSF-1R peaks (top) and on gene transcription starting sites (TSS) of CSF-1R, H3K4me1, EGR1 and H3K4me3 peaks in monocytes. d Peak calling for H3K4me1 (orange), H3K4me3 (purple), EGR1 (red), CSF-1R (blue). ChIP-seq on PU.1 and KLF6 genes in monocytes (TSS: transcription starting site, TTS: transcription termination site). e Peak calling on PU.1 and CEBPD genes for EGR1 in monocytes and for CSF-1R in monocytes (Mo) from two donors (in blue, same donors as in Fig. 3), wild-type THP-1 clones (THP1/WT), THP-1 clones deleted for EGR1 (pool of 3 clones, THP1/CRISPR)