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. 2019 Apr 26;10:1935. doi: 10.1038/s41467-019-09970-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — CSF-1R negatively regulates PU.1 gene expression in human monocytes. a The expression of indicated genes was measured by RT-qPCR in monocytes transfected 72 h before with a scramble (SCR) or a CFMS specific siRNA (mean +/− SEM of 6 independent experiments, results in CFMS-siRNA transfected cells normalized to SCR-siRNA = blue hatched line; Mann–Whitney test: *P < 0.05; ns, non significant). b Expression of LUCIFERASE (LUC, normalized to Renilla) and CFMS (normalized to TUBULIN) genes in monocytes, 48 h after transfection with a scramble (SCR) or a CFMS specific siRNA and PGL4.17-EGR1 motif plasmid (mean +/− SEM of 3 independent experiments, results in CFMS-siRNA transfected cells normalized to SCR-siRNA = blue hatched line; Paired t test: *P < 0.05, **P < 0.01). c Expression of LUCIFERASE (normalized to Renilla) and CFMS (normalized to TUBULIN) in monocytes 48 h after transfection with SCR or CFMS specific siRNA and PGL4.17-PU1 promoter 1 (complete) or PGL4.17-PU1 promoter 2 (deleted of CSF-1R recruitment site) plasmids (mean +/− SEM of 3 independent experiments, normalization to control siRNA with PU.1 promoter 1 = blue hatched line, Paired t test: *P < 0.05, **P < 0.01). d Expression of PU.1 and CFMS genes in monocytes 72 h after transfection with SCR or CFMS specific siRNA (mean +/− SEM of 4 independent experiments, normalization to control siRNA, Paired t test: *P < 0.05)