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. 2019 Apr 26;10:1935. doi: 10.1038/s41467-019-09970-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Differential CSF-1R chromatin localization in CSF-1-induced macrophages. a Nuclear proteins were extracted from monocytes stimulated with 100 ng/mL CSF-1 during the indicated times, and analyzed by immunoblotting for CSF-1R, Lamin B (nucleus marker) and α-tubulin (cytosol marker) (n = 3). b Monocytes were stimulated or not with 100 ng/mL CSF-1 during the indicated times, stained with an anti-CSF-1R antibody (Cter sc-692) or a control IgG and Dapi, followed by confocal imaging analysis of CSF-1R nuclear localization (MFI, mean fluorescence intensity, A.U. arbitrary units, mean +/− SD, Paired t test: ***P < 0.001, n = 1). c Monocytes were treated 3 days with 100 ng/mL CSF-1, fixed and stained for CSF-1R or control IgG (green) and Dapi (blue) followed by confocal imaging (n = 3, scale: 10 μm). d Quantification of the signal generated by CSF-1R labeling (green) and Dapi labeling (blue) according to indicated axes (A.U.: arbitrary units, ROI: region of interest, scale: 10 μm). e Macrophages were stained with an anti-CSF-1R antibody (C-ter sc-692) and Dapi (blue), followed by confocal imaging analysis (stack of 50 pictures of 0.2 μm) to reconstitute an orthogonal view (scale: 10 μm). f Monocytes were differentiated 3 days with 100 ng/mL CSF-1, fixed and stained for CSF-1R (sc-692) followed by electron microscopy (n = 1, scale: 500 nm)