Table 1.
Primer sequences used for RT-qPCR
| Target gene (HUGO nomenclature) | Primers (5′-3′) | Fragment size/bp |
|---|---|---|
| RUNX2 |
F: CCTCCTACCTGAGCCAGATG R: ATGAAATGCTTGGGAACTGC |
165 |
| ALP (ALPL) |
F: ACCATTCCCACGTCTTCACATTTG R: AGACATTCTCTCGTTCACCGCC |
162 |
| COL1 (COL1A1) |
F: AGGGACACAGAGGTTTCAGT R: AGCACCATCATTTCCACGAG |
188 |
| PHEX |
F: TATCTTGAAGCTGGACCAAGCA R: ACTTGTAAAGGGCATCCCGA |
101 |
| MEPE |
F: GGGCCTGCCCATTCCTTCTCGT R: ACCCCAGGAGCCTTTCCCTTGTG |
178 |
| PSA (KLK3) |
F: AGTGCGAGAAGCATTCCCAAC R: CCAGCAAGATCACGCTTTTGTT |
138 |
| AR |
F: CTGGACACGACAACAACCAG R: CAGATCAGGGGCGAAGTAGA |
245 |
| BMP1 |
F: CTTCTGGCACGAACACACTCG R: ACTCCTGCCCTGGCTGGAT |
80 |
| ON (SPARC) |
F: CAAATACATCCCCCCTTGCC R: GATCTTCTTCACCCGCAGCTT |
151 |
| OPG (TNFRSF11B) |
F: TTCCGGAAACAGTGAATCAA R: CGCTGTTTTCACAGAGGTCA |
287 |
| FN (FN1) |
F: CAGTGGGAGACCTCGAGAAG R: TCCCTCGGAACATCAGAAAC |
168 |
| PTH1R |
F: GCGGAGCTGAGGAGACGTAG R: CTTCCCTGATGTGGACGCAG |
185 |
| SOST |
F: AGAGTACCCCGAGCCTCC R: AGCTGTACTCGGACACGTCTTTG |
116 |
| IBSP |
F: ACGAACAAGGCATAAACGGCACCA R: CTTGCCCTGCCTTCCGGTCT |
153 |
| E11 (PDPN) |
F: TTACTAGCCATCGGCTTCATTG R: GGCGAGTACCTTCCCGACAT |
71 |
| RANKL (TNFSF11) |
F: CTCAGCCTTTTGCTCATCTCACT R: CAAGAGGACAGACTCACTTTATGG |
77 |
| DDC |
F: CAAGTCACTCCCGGCTGC R: CTCCTTCCCTCTCCTTCGGA |
84 |
| ENO2 |
F: GTGCACAGGCCAGATCAAGA R: ACAGCACACTGGGATTACGG |
140 |
| RPL32 |
F: GCACCAGTCAGACCGATATG R: ACTGGGCAGCATGTGCTTTG |
151 |
| 7SL |
F: ATCGGGTGTCCGCACTAAGTT R: CAGCACGGGAGTTTTGACCT |
126 |