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MALDI-TOF spectrum from ligation of dimer 1cg to primer 3 under optimized conditions after 10 d reaction time. Conditions: 30 μM primer, 2 mM dimer, 40 μM template 5gc, 40 μM helper 8, 0.5 M MOPS, 0.8 M EDC, 0.15 M 1-EtIm and 0.08 M MgCl2 at pH 6.0 and 4°C. The low level side product observed at a mass 155 Da above that of the extended primer is probably from a side reaction with EDC.
