Table 1.

Results of ligations of dimer 1ug to primer 3 on template 5ac, with or without helper 8 and different reaction conditions^a

Oligomers	pH	T [°C]	Buffer	Conversion [%]b
5ac/3	7.5	22	HEPES	9
5ac/3	6.0	22	HEPES	19
5ac/3/8	6.0	22	HEPES	24
5ac/3/8	6.0	4	HEPES	49
5ac/3/8	6.0	4	MOPS	68
5ac/3/8	6.0c	4	None	71

^aConditions: 10 mM dimer, 40±10 μM primer, 60±15 μM template, 60±15 μM helper, 500 mM HEPES or MOPS, 800 mM EDC, 150 mM 1-ethylimidazole and 80 mM MgCl₂.

^bAs determined after ≤6 days.

^cStarting value.