Table 2.

Conversion of the primer in extension assays with dimers of different sequence^a

Sequences	Dimer/trimer	Concentration dimer/trimer [mM]	Conversion [%]b
6uu/4/9	1aa	2	22
6gu/4/9	1ca	2	25
6cu/4/9	1ga	2	10
6au/4/9	1ua	2	56
6ug/4/9	1ac	2	11
6gg/4/9	1cc	2	13
6cg/4/9	1gc	2	28
6ag/4/9	1uc	2	25
6uc/4/9	1ag	2	47
6gc/4/9	1cg	2	65
6cc/4/9	1gg	2	75
6ac/4/9	1ug	2	83
6ua/4/9	1au	5	13
6ga/4/9	1cu	5	19
6ca/4/9	1gu	5	22
6aa/4/9	1uu	5	3
7uuu/4/8	2aaa	1.0	41
7uuu/4/8	2aaa	0.1	17
7ggg/4/8	2ccc	0.1	59

^aConditions: 30 μM primer, 45 μM template, 45 μM helper, 500 mM MOPS, 800 mM EDC, 150 mM 1-EtIm, 80 mM MgCl₂, pH 6.0 and 4°C.

^bAs detected by relative peak intensities in MALDI-TOF mass spectra after 20 days, correction factors for lower desorption/ionization yield were applied for dimers with 3′-terminal G and trimers, and data are uncorrected in the other cases.