Figure 4.

(A) Effect of SHMT1 on UTR2 levels in H1299 lung cancer cells. Expression of shmt1 and UTR2 RNAs, measured 48 h after transfection with the iscramble (iscr) or ishmt1 (is1). (B) Effect of SHMT1 on the translation of UTR2-containing luciferase reporter mRNA in H1299 lung cancer cells. The panel shows the ratio between firefly and Renilla luciferase evaluated 24 h after transfection with the indicated plasmids (empty pGL3 or pGL3 containing UTR2) and the indicated RNAis. Reduction of SHMT1 by interference increases the amount of translation from UTR2-containing transcripts (UTR2 is1) with respect to control (UTR2 iscr). Statistical analysis is performed on three independent experiments. * P ≤ 0.05 ** P ≤ 0.01