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. 2019 Feb 27;47(8):4240–4254. doi: 10.1093/nar/gkz129

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Effect of SHMT substrates on the binding of UTR2. EMSA carried out by incubating 5.4 μM of SHMT1 with the indicated amounts of 5-CHO-THF-Glu₅ in absence (A) or presence (B) of 10 mM glycine prior to the addition of 0.18 μM unlabelled UTR2. (C) Effects of glycine concentration on the folate-mediated displacement of UTR2. EMSA was performed incubating 5.4 μM of SHMT1 with the indicated amounts of glycine and a fixed concentration of 5-CHO-THF-Glu₅ (27 μM) prior to the addition of 0.18 μM unlabelled UTR2.