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. 2019 Mar 1;47(8):3937–3956. doi: 10.1093/nar/gkz128

Figure 5.

Figure 5.

Benign PNT2C2 prostate cells express less POLR3G and are less sensitive to ML-60218 than PC-3 cells. (A) Normalized relative levels of POLR3A, POLR3G and POLR3GL mRNAs in PNT2C2 and PC-3 cells, as determined by RT-qPCR in two independent experiments. (B) Western blot of POLR3A, POLR3G, POLR3GL and actin proteins in PC-3 and PNT2C2 cells. (C) Means of the relative changes in normalized expression of unprocessed pre-tRNATyr and mRNAs encoding CK8, CK14, CK18, SYP, NSE, GRP78, CD55, CD59, CD63 and NANOG in PNT2C2 cells harvested 48 hrs after treatment with or without 20μM ML-60218, as determined by RT-qPCR in three independent experiments. (D) Means of PNT2C2 cell numbers over 3 days in presence or absence of 20μM ML-60218, in three independent experiments. (E) Western blot of POLR3G and actin in PNT2C2 cells harvested 48 h after transfection with control or POLR3G-targeting siRNAs. (F) Means of PNT2C2 cell numbers over 3 days following transfection with control or POLR3G-targeting siRNAs, as indicated, in three independent experiments. (G) Means of the percentage of viable PNT2C2 and PC-3 cells, as determined using Alamar blue, after 2 days exposure to the indicated concentrations of ML-60218, in three independent experiments. * indicates P < 0.05 relative to control by t-test; *** indicates P < 0.005. Error bars represent S.E.M.