Table 2.
Primers used for real-time PCR
| Transcript | Primers | Accession no./locus |
|---|---|---|
| pre-tRNATyr | FWD – CCTTCGATAGCTCAGCTGGTAGAGCGGAGG | chr14:21128117-21128210 |
| REV - CGGAATTGAACCAGCGACCTAAGGATCTCC | ||
| Chr16.tRNA1 | FWD – ATACAGCCGCAGGGCC | chr16:3200675-3200747 |
| REV – CGTTAACATGGCAACGAC | ||
| Chr16.tRNA114 | FWD – GGATCCTGTGGTGACCCA | chr17:66016013-66016085 |
| REV – CTAATCTCACGCGACCCAGATG | ||
| ARPP P0 | FWD – GCACTGGAAGTCCAACTACTTC | NM_001002.4 |
| REV – TGAGGTCCTCCTTGGTGAACAC | ||
| CD55 | FWD – GATGTACCTAATGCCCAGCCAGC | NM_000574.4 |
| REV – CATGAGAAGGAGATGGTTGCACC | ||
| CD59 | FWD – AAGGAGGGTCTGTCCTGTTCGG | NM_000611.5 |
| REV – GGATGAAGGCTCCAGGCTGCT | ||
| CD63 | FWD – GTGCAGTGGGACTGATTGCCGT | NM_001257389.1 |
| REV – GGGACTCGGTTCTTCGACATGG | ||
| Cytokeratin 8 (CK8) | FWD – CGGAATGAATGGGGTGAG | NM_001256282.1 |
| REV – TGGTAGAGGCAGGAGTGGAG | ||
| Cytokeratin 14 (CK14) | FWD – CCTCCTCCCAGTTCTCCTCT | NM_000526.5 |
| REV – ATCGTGCACATCCATGACC | ||
| Cytokeratin 18 (CK18) | FWD – TGATGACACCAATATCACACGA | NM_000224.3 |
| REV – GGGCTTGTAGGCCTTTTACTTC | ||
| DR2 Alu | FWD – CCTGAGGTCAGAAGTTCAAGACC | Multiple loci |
| REV – GCAATGGCATGGTCTCAGCTC | ||
| Chr2.tRNA6 | FWD – ACGCACTCTAGCCTGTGGT | chr2:75124046-75124118 |
| REV – ACTGGTTCCCTGACCAG | ||
| Chr2.tRNA20 | FWD – CCTTTCAGCAGTTCCCATA | chr2:131094701-131094772 |
| REV – GCCACTTTTGTTCCCATACC | ||
| Chr1.tRNA84 | FWD – TGTCACCAGCCCTCCCT | chr1:161391883-161391954 |
| REV - GCAAACTTCCCTGACCG | ||
| GRP78 | FWD – GCTGAAGACAAGGGTACAGGGAAC | NM_005347.5 |
| REV – GGAGGGCCTGCACTTCCATAGA | ||
| NANOG-Alu-Sx | FWD – GGGACTCGGTTCTTCGACATGG | chr12; 7783172–7783477 |
| REV – GGCTGATCTTGAACTCCCG | ||
| NANOG mRNA | FWD – GATTTGTGGGCCTGAAGAAA | NM_024865.4 |
| REV – AAGTGGGTTGTTTGCCTTTG | ||
| Synaptophysin (SYP) | FWD – CCAATCAGATGTAGTCTGGTCAGT | NM_003179.2 |
| REV – AGGCCTTCTCCTGAGCTCTT | ||
| Neuron specific | FWD – CAATGTGGGGGATGAAGG | NM_001975.3 |
| enolase (NSE) | REV – GTGTAGCCAGCCTTGTCGAT | |
| POLR3G | FWD – CACTTCGGCTGCAGAGTTTT | NM_006467.3 |
| REV – AGTGGGCAAATTCTGAAAG | ||
| POLR3GL | FWD – AGAGCTACGAGGAGCCATGA | NM_032305.3 |
| REV – TTGTCTGAATAACGCTCCACA | ||
| POLR3A | FWD – TCTGGAGACCTGTAGGGACAA | NM_007055.4. |
| REV - CTGGCTCACCAATGCTCT | ||
| TERT | FWD – GCCTTCAAGAGCCACGTC | NM_198253.2 |
| REV – CCACGAACTGTCGCATGT | ||
| TERC | FWD – CGAGGTTCAGGCCTTTCA | NR_001566.1 |
| REV – CCACAGCTCAGGGAATCG |