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. 2019 Feb 4;47(8):4054–4067. doi: 10.1093/nar/gkz066

Figure 2.

Figure 2.

nup84Δ sensitivity to UV is exacerbated during S phase independently of SUMO homeostasis. (A) Sensitivity of WT (BY4741), rad7Δ (yHG72-1B), nup84Δ (YDL116W) and nup84Δ rad7Δ (yN84R7-24A) cells with and without expression of a GFP-Nsp1-Ulp1C fusion protein to 4-NQO, UV light, MMS and HU. 10-fold serial dilutions of exponentially growing cells are shown. (B) UV survival curves of asynchronously growing (dash lines) or early S phase-enriched (S; plain lanes) WT (BY4741), nup84Δ (YDL116W), rad1Δ (YPL022W) and rad52Δ (YML032C) cells. Average and standard deviation are plotted (N ≥ 3). Statistical analyses were performed between both growth conditions for each genotype. **P < 0.01; ***P < 0.001 (Two-way ANOVA). (C) UV survival curves of asynchronously growing (dash lines) or early S phase-enriched (S; plain lanes) WT (BY4741) and nup84Δ (YDL116W) cells transformed with an empty vector (–) or a plasmid expressing the GFP-Nsp1-Ulp1C fusion protein (N ≥ 3). Statistical analyses were performed between GFP-Nsp1-Ulp1C expression and empty vector for each genotype and growth condition. *P < 0.05. Other details as in B.