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. 2019 Feb 4;47(8):4054–4067. doi: 10.1093/nar/gkz066

Figure 4.

Figure 4.

Replication-born DSBs are particularly harmful in the absence of Nup84. (A) Sensitivity of wild type (WT, WRB1-1A), nup84Δ (WRN84B1-10C), rad53K227A (CY7031) and nup84Δ rad53K227A (yHG159-15C) to 4-NQO, UV, MMS and HU. 10-fold serial dilutions of exponentially growing cells are shown. (B) Western blot analysis of Rad53 in wild-type (WT, BY4741) and nup84Δ (YDL116W) cell cultures. HU-treated cells (50 mM HU for 2 h) are shown as control of Rad53 phosphorylation. (C) Sensitivity of wild type (WT, WRB1-1A), nup84Δ (WRN84B1α-3D), rad3-102 (yHG164-9D) and nup84Δ rad3-102 (yHG164-9A) to 4-NQO and UV. 10-fold serial dilutions of exponentially growing cells are shown.