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. 2019 Feb 22;47(8):3888–3903. doi: 10.1093/nar/gkz119

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — SET2Δ3 rescues H3K36 methylation in set2 mutants lacking the SRI or HB domains. (A) The schematic depicts Set2 and a set of mutants missing the indicated domains, testing each for H3K36me2 and H3K36me3 levels by western analysis. The red line in the AID represents the position of the SET2Δ3 mutation. The numbers next to each mutation show the levels of H3K36me2 and H3K36me3 in each mutant strain relative to a wild-type control (mean ± standard deviation of at least three experiments). (B) ChIP-qPCR assaying localization of FLAG-tagged Set2 wild-type and mutant proteins at the LSC2, RIX1 and PUN1 genes. The black dots represent the individual data points for three experiments, and the bars show the mean ± standard deviation. ChIP-qPCR data have been spike-in normalized to the Schizosaccharomyces pombe ACT1 gene. (C) Spot tests of cells grown at 37°C, assaying expression of the FLO8-URA3 reporter in the indicated strains.