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. 2019 Apr 25;19(5):669–674. doi: 10.1089/ast.2018.1935

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© Hyo-Joong Kim and Justin Kim, 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 2. — The coupling of ribose 1,2-cyclic phosphate 3 and cytosine provides cytidine 2′-phosphate 5. (A) Synthetic scheme of the coupling of ribose 1,2-cyclic phosphate 3 and cytosine to give cytidine 2′-phosphate 5. (B) HPLC trace of coupling of ribose 1,2-cyclic phosphate 3 and cytosine and its expanded view. (C) ¹H NMR spectrum of the HPLC purified (collected fraction from 8 to 9 min) fraction of the coupling of 3 and cytosine, which shows the existence of cytidine 2′-phosphate 5 as a major component.