FIG. 3.

MyD88^−/− MSCs did not activate stat3 in response to LPS stimulation and exhibited a slower rate of proliferation. WT and MyD88^−/− MSCs were stimulated with or without LPS (200 ng/mL) for 24 h. Phosphorylated stat3 levels in protein extracts from all groups were analyzed by western blot. (A) Representative blot is shown. Three technical replicates per group are presented. (B) Western signals from images of scanned X-ray films were analyzed, normalized to GAPDH, and quantified as pixel densities. (C) Fifteen thousand cells from both genotypes were plated per well in six-well plates. Cell numbers were counted at 5 days or 8 days. *P < 0.05 versus control MSCs as determined by one-way ANOVA followed by Tukey post hoc test.