Figure 2.

RNA gel-blot analysis of MeJA-induced Atvsp gene expression in leaves of Col, jar1-1, RLD, and Ws. Five-week-old plants were treated by dipping the leaves in a solution containing 0 or 100 μm MeJA. Two days after MeJA-treatment the leaves were harvested. An Arabidopsis Atvsp gene-specific probe was used for the RNA gel-blot hybridization. To check for equal loading, the blot was stripped and hybridized with an Arabidopsis gene-specific probe for β-tubulin (Tub). The experiment was repeated with similar results.