Table I.
Genetic segregation of triple response expression at 0.5 μm ACC in Col, RLD, and F2 plants of the RLD × Col crossa
| Genotype | Treatment (μM ACC) | Total No. of Seedlings | Triple Responseb | No Triple Responsec | Expected Ratio | χ2 value | P |
|---|---|---|---|---|---|---|---|
| Col | 0 | 48 | 0 | 48 | 0:1 | – | – |
| 0.5 | 56 | 53 | 3 | 1:0 | – | – | |
| RLD | 0 | 52 | 3 | 49 | 0:1 | – | – |
| 0.5 | 53 | 4 | 49 | 1:0 | – | – | |
| F2 | 0 | 52 | 4 | 48 | 0:1 | – | – |
| 0.5 | 74 | 54 | 20 | 3:1 | 0.16 | 0.69 |
a
Surface-sterilized seeds were plated onto MS-agar plates with or without 0.5 μm ACC. After germination for 2 d at 4°C, seedlings were grown for 5 d at 20°C in the dark and examined for triple-response expression. The experiment was repeated twice, yielding similar results.
b
No. of seedlings exhibiting triple response characteristics.
c
No. of seedlings showing no triple response characteristics.