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. 2019 Apr 10;116(17):8493–8498. doi: 10.1073/pnas.1818522116

Fig. 1.

Fig. 1.

Bacterial clearance and alveolar epithelial damage and repair in SpT4-infected mice. Lung tissues were collected on 2, 7, 14, and 30 dpi with SpT4. (A) H&E staining. (B) Immunostaining with antibodies to the type 4 capsular of SpT4 (green). (C) Bacteria loads in lung homogenate measured by cfu plating (LOD: limit of detection). (D and F) Immunostaining with mAb to T1α (D, green), SPC (F, red), and CC10 (F, green). DAPI: blue in B, D, and F. [Scale bar: 500 μm (A); 50 μm (B, D, and F).] (E and G) qRT-PCR of T1α and SPC mRNA in lung tissue. Data are mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 versus noninfected normal lungs (0 dpi).