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. 2019 Apr 10;116(17):8493–8498. doi: 10.1073/pnas.1818522116

Fig. 3.

Fig. 3.

Effects of miR-302b/c mimics treatment on SpT4-infected mice. (A) Schematic of experimental design. Mice were infected with SpT4 on day 0 and then treated with either miR-302b/c or Ctrl mimics at 5 and 6 dpi and monitored daily for survival (B), gain of body weight (C), and blood oxygen levels (F). Total protein levels (D) and LDH activities (E) in BALF at indicated dpi. Pulmonary functions (G) were analyzed at 21 dpi for compliance, forced expiratory volume in 0.1 s (FEV0.1), and forced vital capacity (FVC). (H) Immunostaining of lung sections with antibodies to T1α and to SPC. (Scale bars: 50 µm.) (I) qRT-PCR of T1α and SPC mRNA in lung tissues. Normal: uninfected/untreated control. Data in B were analyzed using the Gehan–Breslow–Wilcoxon test of cumulative data (n = 14 for miR-302b/c; n = 16 for Ctrl mimics). Data shown are means ± SEM (C–G, n = 4 per group; I, n = 10 per group). *P < 0.05; **P < 0.01; ***P < 0.001.