Fig. 4.

AEC proliferation following miR-302b/c mimics treatment of SpT4-infected mice. (A) Schematic of experimental design. (B and C) Confocal images of lung sections at 7 dpi by Click-iT EdU Alexa Fluor 488 imaging and coimmunostaining with antibodies to SPC (AECII, B) and to Hopx (AECI, C), and quantification of EdU⁺SPC⁺ and EdU⁺Hopx⁺ cells as percentage of total SPC⁺ and Hopx⁺ cells, respectively. Arrowheads in B and C point to nucleus of proliferating (EdU⁺) cells. Quantitative analyses represent counting of multiple fields from five independent samples per group (∼2,200 SPC⁺ cells and ∼765 Hopx⁺ cell per sample). Data are means ± SEM (n = 5–6 per group). (D) Expression of indicated genes of interest by qRT-PCR analysis of the mRNA of isolated lung epithelial cells at 7, 14, and 21 dpi. Data are means ± SEM (n = 3 per group and time point). *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t test). (Scale bars: 10 µm.)